Additional Selective and Differential Cell Culture Media
NEOGEN OGYE Agar Base 5 kg
OGYE Agar Base is used with oxytetracycline in the detection and isolation of yeasts and molds from foods in a laboratory setting. OGYE Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Mossel, Visser, and Mengerink described Oxytetracycline-Glucose Yeast Extract (OGYE) Agar for the detection and enumeration of yeasts and molds in foods and other specimens of sanitary significance. Mossel et al. described improved recovery of yeasts and molds compared to acidified agar. This medium is supplemented with oxytetracycline as a selective agent, rather than relying on the low pH of acidified agar to suppress bacterial growth. Acidified agar is commonly used for detection of yeasts and molds in foods and dairy products, and OGYE Agar Base provides an alternate medium. For more information visit https://www.neogen.com/solutions/Microbiology/ogye-agar-base
NEOGEN Pancreatic Digest of Gelatin, 5 kg
Pancreatic Digest of Gelatin is an enzymatic digest of gelatin for use in preparing microbiological culture media in a laboratory setting. Pancreatic Digest of Gelatin is not intended for use in the diagnosis of disease or other conditions in humans.Pancreatic Digest of Gelatin provides a complex nitrogen source and is used as a media ingredient for fermentation studies. Pancreatic Digest of Gelatin alone is used to support the growth of non-fastidious microorganisms. Pancreatic Digest of Gelatin is deficient in carbohydrates and distinguished by low cystine and tryptophan content.For more information visit https://www.neogen.com/solutions/Microbiology/pancreatic-digest-gelatin
NEOGEN Tetrathionate (TT) Broth Base, Hajna, 10 kg
Tetrathionate (TT) Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella species (except S. typhi) from samples in a laboratory setting. Tetrathionate (TT) Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base, Hajna, is recommended in the Compendium of Methods for the Microbiological Examination of Foods and the FDA/BAM for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and whole eggs.. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Numerous reported cases of Salmonella enteritidis (SE) infections are associated with contaminated shell eggs.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-tt-broth-base-hajna
NEOGEN Glucose OF Medium, 5 kg
Glucose OF Medium is used for the confirmation of Enterobacteriaceae in foods according to ISO 21528-1&2:2017, and is not intended for use in the diagnosis of disease or other conditions in humans.Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bromothymol blue is the pH indicator.Colonies of presumptive Enterobacteriaceae should be confirmed by means of tests for the fermentation of glucose and the presence of oxidase. If the colonies are oxidase-negative and glucose-positive, the sample shall be regarded as being positive for Enterobacteriaceae.For more information visit https://www.neogen.com/solutions/Microbiology/glucose-of-medium
NEOGEN Potato Dextrose Broth, 10 kg
Potato Dextrose Broth is used for the cultivation of fungi in a laboratory setting. Potato Dextrose Broth is not intended for use in the diagnosis of disease or other conditions in humans. Potato Dextrose Broth is a general purpose broth for yeasts and molds. The low pH of this medium inhibits bacterial growth. Potato Dextrose Broth is the same formula as Potato Dextrose Agar, but agar has been omitted.For more information visit https://www.neogen.com/solutions/Microbiology/potato-dextrose-broth
NEOGEN Buffered Listeria Enrichment Broth Base, 10 kg
Buffered Listeria Enrichment Broth Base is used with supplements for selective enrichment of Listeria spp. in foods and is not intended for use in the diagnosis of human disease.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth-base
NEOGEN MacConkey Agar No. 3, 5 kg
MacConkey Agar No. 3 is used for the cultivation of Enterobacteriaceae and is not intended for use in the diagnosis of disease or other conditions in humans.A modification recommended by the W.H.O. and the American Public Health Association for the isolation of Enterobacteriaceae from waters and sewage. The medium has been made more selective than MacConkey’s original formula by the use of crystal violet as well as bile salts. Gram positive organisms will not grow on this medium.Formula:Peptone - 20.0 g/LLactose - 10.0 g/LBile Salt No.3 - 1.5 g/LSodium Chloride - 5.0 g/LNeutral Red - 0.03 g/LCrystal Violet - 0.001 g/LAgar - 15.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/macconkey-agar-number-3
NEOGEN Casein Peptone Type I, 5 kg
Casein Peptone Type I is an enzymatic hydrolysate of casein for use in preparing microbiological culture media in a laboratory setting and is not intended for use in the diagnosis of disease or other conditions in humans.Casein Peptone Type I is an enzymatic hydrolysate of casein, rich in peptones and amino acids (including tryptophane). This peptone can be utilized by most bacteria as a growth substrate.For more information visit https://www.neogen.com/solutions/Microbiology/casein-peptone-type-1
NEOGEN D/E Neutralizing Broth without Tween, 10 kg
D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth without Tween is not intended for use in the diagnosis of disease or other conditions in humans.D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.For more information visit https://www.neogen.com/solutions/Microbiology/de-neutralizing-broth-without-tween
NEOGEN Clostridium Difficile Agar Base, 500 g
Clostridium Difficile Agar Base (Brazier’s) is used with blood, Cycloserine, and Cefoxitin for the isolation of Clostridium difficile. Clostridium Difficile is not intended for use in the diagnosis of disease or other conditions in humans.Brazier’s CCEY agar is the formulation currently used by the Anaerobe Reference Unit for the isolation of C. difficile, resulting from work initiated by Ken Phillips and Paul Levett, and completed by Jon Brazier. Based upon the market leading anaerobe medium, Fastidious Anaerobe Agar, it incorporates additional ingredients to improve the isolation and differentiation of C. difficile from samples.Cholic acid is present to promote spore germination following alcohol shock treatment, and p-hydroxyphenylacetic acid to enhance the production of p-cresol, a distinctive metabolite of C. difficile.For more information visit https://www.neogen.com/solutions/Microbiology/clostridium-difficile-agar-base
NEOGEN Tryptose, 500 g
Tryptose is an enzymatic digest of protein for use in preparing microbiological culture media in a laboratory setting. Tryptose is not intended for use in the diagnosis of disease or other conditions in humans. Tryptose was developed while studying growth requirements of Brucella spp. Huddleson found Tryptose media to be equal or superior to meat infusion media, providing uniformity for the cultivation and differentiation of fastidious organisms including Neisseria gonorrhoeae, Streptococcus milleri and Brucella spp.. Casman reported that a medium consisting of 2% Tryptose, along with other media ingredients, equaled fresh beef infusion base with respect to organism growth. Tryptose is recommended in broth and agar formulations, and when used in media containing blood to permit hemolytic reactions. Tryptose is used in the preparation of Tryptose Broth (Product Code No. NCM0087).For more information visit https://www.neogen.com/solutions/Microbiology/tryptose
NEOGEN Sorbitol MacConkey Agar, 5 kg
Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases. MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.For more information visit https://www.neogen.com/solutions/Microbiology/sorbitol-macconkey-agar
NEOGEN Campy Blood-Free Selective Medium (Modified CCDA) (EMEA), 500 g
Campylobacter Blood-Free Selective Medium (Modified Charcoal Cefoperazone Deoycholate Agar or mCCDA) is used for the cultivation of campylobacters, and is not intended for use in the diagnosis of disease or other conditions in humans.A blood free medium which will support the growth of most enteric campylobacters. The selective supplement X112 (X212) makes the medium selective for C. jejuni. and C. coli when incubated at 37°C. With this product incubation at 42°C is no longer necessary and higher recovery rates have been reported at 37°C than at 42°C. The supplement X112 (X212) consists of cefoperazone and amphotericin and is superior to the selective combination of Skirrow, Butzler and Blazer-Wang all of which contain antibiotics shown to be inhibitors to C. coli. The colonial morphologies of Campylobacter spp. on this medium are distinctive.For more information visit https://www.neogen.com/solutions/Microbiology/campy-blood-free-selective-medium-modified-ccda-emea
NEOGEN Slanetz and Bartley Agar, 500 g
Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium was originally described by Slanetz and Bartley for the enumeration of enterococci from water samples using a membrane filtration technique, but it may also be used as a spread plate for the examination of other sample types. Enterococci reduce tetrazolium chloride to the insoluble red dye formazan, producing colonies which are dark red or maroon on the surface of the membrane or agar. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive. Colonies may be confirmed as enterococci by demonstrating aesculin hydrolysis using Kanamycin Aesculin Azide Agar (NCM0198).For more information visit https://www.neogen.com/solutions/Microbiology/slanetz-bartley-medium
NEOGEN Agar, Bacteriological No. 2, 10 kg
Agar, Bacteriological No. 2 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-bacteriological-number-2